Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients in wasit province, Iraqin wasit province, Iraq

ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment devices is a significant cause nosocomial infections. In this study, total 100 clinical specimens (urine, sputum pus) were collected from patients admitted in the ICU. Results showed eighty two positive growth culture. 30(36.5%) female and‎‏‎ 52( 63.5%) male ‎ to intensive care unit distributed 69 (84.1%) gram-‎negative bacteria .while gram 13(15.8%), as well 20 pure ‎isolates some places, materials 12 (75%) ‎Gram negative .While 8 (25%) it bacteria. antibiotic susceptibility test only Trimethoprime was effective choice against Acinetobacter baumannii. Phenotypically survey carbapenem klebsiella pneumoniae ‎were 9(47%), pseudomonas aeruginosa‏ ‏was 7(35%‎‏ MBL Carbapenemase result E. Coli 7(43%) similar ‎results (40%). Whereas, Genotypically most prevalent gene E.Coli 1 (10%), Burkholderia cepacia blaVIM 1(100%), its prevalence 7 (53%) among Klebsiella pneumonia NDM, Pseudomonas aeruginosa 4(33.3%) Escherichia 1(10%), (100%) oxytoca KPC.